Flow Cytometry

Technical specification

ThermoFisher Bigfoot Full Spectrum Cell Sorter

The Bigfoot has an integrated containment cabinet providing sample and operator safety equivalent to Class II containment. 

• 5 laser, 48 fluorescent detector (12UV, 12V, 7B, 12Y, 5R) 
• 6 scatter detectors off the 488 blue laser
• equipped with 355nm, 405nm, 488nm, 565nm and 633nm lasers
• analyse 54 parameters including Forward scatter (FSC) and Side scatter (SSC)

The instrument can sort: 

• up to 6 independent populations into a mixture of tube types including FACS tubes, Eppendorf type tubes, 15ml and 50ml Falcon type tubes
• single cells or many cells into various multi-well plate formats up to and including 1536 well plates

BDFACSAriaIIu Conventional Cell sorter

We have two BDFACSAriaIIu insturments which have:

• 3 lasers
• 9 colour cell sorters 
• 405nm, 488nm and 633nm lasers 
• an Automatic Cell Deposition Unit (ACDU) 

The instrument can: 

• analyse 11 parameters including FSC and SSC
• sort up to 4 independent populations into FACS tubes or Eppendorf type tubes, 
• sort 2 independent populations into 15ml Falcon type tubes
• sort single cells or many cells into various multi-well plate formats up to and including 384 well plates

BDFACSAriaFusion conventional Cell sorter

The BDFACSAriaFusion is located in the WISH lab and has:

• 4 lasers
• 15 colour cell sorter
• 405nm, 488nm, 561nm and 633nm lasers
• an Automatic Cell Deposition Unit (ACDU) 

The instrument can: 

• sort up to 4 independent populations into FACS tubes, Eppendorf type tubes
• sort 2 independent populations into 15ml Falcon type tubes
• can analyse 17 parameters including FSC and SSC
• sort single cells or many cells into various multi-well plate formats up to and including 384 well plates
   

ThermoFisher Attune NxT cytometer

The Attune NxT flow Cytometer located within the School of Human Development and Health and has:

• 3 lasers
• 11 colour analyser
• 405nm, 488nm and 633nm lasers 
• a volumetric sample and sheath fluid delivery system
• acoustic focusing to achieve high sensitivity and high throughput

The instrument can:

• analyse 13 parameters including FSC and SSC 

BDAccuric6 cytometer

The BDAccuriC6 has:

• 2 lasers of 488nm and 633nm

The instrument can detect a total of 6 parameters including FSC and SS

• up to 3 parameters from the blue laser 
• 1 parameter from the red laser 

BDFACSCalibur cytometer

The BDFACSCalibur has:

2 lasers of 488nm and 633nm

The instrument can detect a total of 6 parameters including FSC and SS

• up to 3 parameters from the blue laser 
• 1 parameter from the red laser